Fig. 4

CTT normal (negative) but HRCM positive images in a representative FC patient (Case 54). A CTT normal in Case 54. B HRCM positive in Case 54. C HE staining showed constipation in Case 54. Left, the intermuscular nerve with abnormal morphology was unevenly distributed, and no obvious ganglion and smooth muscle cell edema were observed. Right, nonspecific esterase staining (NSE) showed no neurons in one ganglion