Fig. 4

Nec-1, a RIP3 inhibitor, treatment could relieve intestinal pathology and histopathology in DSS-induced colitis mice. A–D Balb/c wild-type and RIP3 knockout mice freely drank 3% DSS solution for 7 days. On the 8th day, Nec-1 (5 mg/kg), SASP (50 mg/kg) and placebo (1% DMSO) were injected intraperitoneally for 14 consecutive days. A DAI, C the length of mouse colon and D CMDI scores were measured, and B the entire colonic intestine from the anus to the end of the cecum was isolated and photographed. E H&E staining results were imaged under a 20 × objective. Scale bar: 100 μm. The histology scores are shown on the right panel. The data are presented as mean ± SD. The asterisk indicates a significant difference (*P < 0.05) between groups. Abbreviations: DSS, dextran sulfate sodium; SASP, sulfasalazine; DAI, disease activity index; CMDI, colon gross morphological damage index