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Fig. 2 | BMC Gastroenterology

Fig. 2

From: RIP3 knockdown inhibits necroptosis of human intestinal epithelial cells via TLR4/MyD88/NF-κB signaling and ameliorates murine colitis

Fig. 2

Knockdown of RIP3 expression inhibits the pro-apoptotic effect of TNF-α on intestinal epithelial cells. A RT-qPCR demonstrated the knockdown efficiency of shRIP3 plasmid. B MTT assay detected the effect of shRIP3 knockdown plasmid and RIP3 inhibitor GSK872 (2 μM) on cell viability after TNF-α (10 ng/ml, 12 h) treatment. C Annexin V-FITC and PI double staining detected the effect of shRIP3 knockdown plasmid and RIP3 inhibitor GSK872 on cell apoptosis. D Semi-quantitative analysis of cell apoptosis (The sum of the percentages of the first and fourth quadrants). E PI staining detected cell necrosis and the results were observed under fluorescence microscope with a 20 × objective. Blue color: Hochest 33342 stains the nucleus; Green color: PI Dye. Scale bar: 200 μm. The semi-quantitative analysis of immunofluoresence via Image J software (right panel)

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BMC Gastroenterology

ISSN: 1471-230X

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