Fig. 1

The expression of RIP3 in the colon is positively proportional to the severity of UC. A The Human Protein Atlas (https://www.proteinatlas.org/search/RIPK3) analyzed RNA expression of RIP3 in more than 60 human tissues and organs. Interestingly, among more than 60 human tissues and organs, RIP3 has the most RNA expression in small intestines. We have shown the RNA expression of RIP3 in Duodenum, Small intestine, Colon, and Rectum. B Western blot detected the expression levels of RIP3 and MLKL in the colon tissues from patients with mild (I), moderate (II) and severe (III) UC based on the modified Truelove & Witts severity classification. The semi-quantitative analysis of western blotting via Image J software. RIP3: *P < 0.05, ~ versus Mild; MLKL: ^#P < 0.05, ~ versus Mild. C Immunohistochemistry detected the expression and distribution of RIP3 and MLKL in the colon tissues from patients with mild, moderate and severe UC. D–E The mean optical density value (IOD) of the target protein in each field was analyzed by Image-Pro Plus 6.0 software. Scale bar: 100 μm. The data were presented as mean ± SD. The asterisk indicates a significant difference (*P < 0.05, **P < 0.01) between groups. Abbreviations: n.s., no significance