Table 1 Primers used in the study
From: Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1 polarization
| Gene name | Primer sequence |
|---|---|
| CTSS |
Forward:5′-TAAGATGGCTGTTTTGGATG Reverse:5′-TTCTTTTCCCAGATGAGACGC |
| IL-1β | Forward:5′-ATGATGGCTTATTACAGTGGCAA Reverse:5′-GTCGGAGATTCGTAGCTGGA |
| TNF-α | Forward:5′-GAGGCCAAGCCCTGGTATG Reverse:5′-CGGGCCGATTGATCTCAGC |
| IL-10 |
Forward:5′-AGAAGGACCAGCTGGACAACAT Reverse:5′-CAAGTAACCCTTAAAGTCCTGCAGTA |
| Caspase-1 |
Forward:5′- TTTCCGCAAGGTTCGATTTTCA Reverse:5′- GGCATCTGCGCTCTACCATC |
| Trypsinogen |
Forward:5′-CATGAATCTACTCCTGATCC Reverse:5′- TGTCATTGTCCAGAGTCC |
| GSDMD |
Forward:5′-CCAGCATG GAAGCCTTAGA G Reverse:5′-CAGAGTCGAGCACCAGACAC |
| CD11c |
Forward:5′-TTC TTC TGC TGT TGG GGT TTG Reverse:5′-CAA CCA CCA CCC AGG AAC TAT |
| CD206 | Forward:5′-CTACAAGGGATCGGGTTTATGGA Reverse:5′-TTGGCATTGCCTAGTAGCGTA |
| IRF1 |
Forward:5′-CCCAAGCTTATGCCCATCACTCGGATGC Reverse:5′-CCGCTCGAGCTACGGTGCACAGGGAATGG |
| IRF3 |
Forward:5′-ACCGACGGCTTTACAGAGAA Reverse:5′-TCCTCGTCACTGCAGTCTTT |
| IRF4 |
Forward:5′-CCATGACAACGCCTTACCCT Reverse:5′-TTATGCTTGGCTCTGTGGGG |
| IRF5 |
Forward:5′-GTTGCCTTTGACGGACCTA Reverse:5′-GTTGCCTTTGACGGACCTA |
| IRF7 |
Forward:5′-ATATCTCACGTGACCGAGGA Reverse:5′-AGCTGATGGTGCTGGAAGTC |
| IRF8 |
Forward:5′-CGAGGTTACGCTGTGCTTTG Reverse:5′-TTATGCTTGGCTCTGTGGGG |
| β-actin |
Forward:5′- GAGACCTTCAACACCCC; Reverse:5′- GTGGTGGTGAAGCTGTAGCC |