Fig. 7
From: Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1 polarization
Macrophage exosome-derived CTSS induced pyroptosis in vivo. HP mice were divided into different groups, normal, HP, HP + PBS, and HP + Z-FL-COCHO. ELISA (A, B) QRT-PCR detection (C) for CTSS and inflammation factor levels in the peripheral blood of the HP mouse model. Proteins level of caspase-1, GSDMD, pro-IL-1β, IL-1β, ASC, and caspase-8 were assessed by western blotting, in HP mice with different treatments (D). Pathologic analysis for HP mice with different treatments, including HE (E) shows the pathology (Scale bars = 200 μm (left); Scale bar = 50 μm (right), and Ki67, immunohistochemistry of GSDMD and caspase-1. Scale bar = 50 μm (F). Data are shown as the mean ± SEM, n = 6, ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001