Fig. 6
From: Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1 polarization
Increased free fatty acids and pancreatic inflammation in a mouse model of hyperlipidemic pancreatitis (HP). A mouse model of HP was constructed in C57BL/6 J mouse (n = 6); and control (n = 6). After 6 weeks, mice were euthanized, and peripheral blood and pancreas tissue were collected. A Amylase and B lipase activity, C Triglyceride and D free fatty acid (FFA) levels in peripheral blood were measured by ELISA. E Plasma inflammation factors (IL-1β, TNF-α, and IL-10) detection by ELISA and F qRT-PCR. Data are shown as the mean ± SEM, n = 6. *P < 0.05, **P < 0.01, ANOVA. CTSS level in HP mouse peripheral blood (G) and pancreas tissue (H). Data are shown as the mean ± SEM, n = 6, ANOVA. **P < 0.01. Western blot (I) of CTSS in HP mouse pancreas tissues. Pathologic analyses were performed in pancreas tissue samples. J Hematoxylin–eosin (HE) staining shows the tissue injury of HP mouse (Scale bars = 200 μm (left); Scale bars = 50 μm (right)), K present the expression of Ki67 and caspase-1 (Scale bars = 50 μm)