Fig. 3
From: Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1 polarization

CTSS is produced in macrophage and transported by exosomes. Western blot analysis of CTSS in macrophage cell lysates and medium after PA (500 μM) stimulation for 24 h (A). Western blot analysis of exosome-specific proteins in exosomes and cell lysates detected 24 h after palmitic acid (PA, 500 μM) stimulation of macrophage (B). Electron microscopy of exosomes (C). (Scale bars = 500 nm) Exosome-derived CTSS from PA (500 μM) stimulated macrophage (D). QRT-PCR (E) and western blot (F) analysis of CTSS gene knockdown efficiency in macrophage. Data are shown as the mean ± SEM, n = 3, ANOVA. *P < 0.05. Detection of CTSS in exosomes from PA (500 μM) stimulated macrophage; macrophage were transfected with shCTSS, scr transfection as the control (G)