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Fig. 3 | BMC Gastroenterology

Fig. 3

From: Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1 polarization

Fig. 3

CTSS is produced in macrophage and transported by exosomes. Western blot analysis of CTSS in macrophage cell lysates and medium after PA (500 μM) stimulation for 24 h (A). Western blot analysis of exosome-specific proteins in exosomes and cell lysates detected 24 h after palmitic acid (PA, 500 μM) stimulation of macrophage (B). Electron microscopy of exosomes (C). (Scale bars = 500 nm) Exosome-derived CTSS from PA (500 μM) stimulated macrophage (D). QRT-PCR (E) and western blot (F) analysis of CTSS gene knockdown efficiency in macrophage. Data are shown as the mean ± SEM, n = 3, ANOVA. *P < 0.05. Detection of CTSS in exosomes from PA (500 μM) stimulated macrophage; macrophage were transfected with shCTSS, scr transfection as the control (G)

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