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Fig. 2 | BMC Gastroenterology

Fig. 2

From: Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1 polarization

Fig. 2

Transcription factor IRF5 is responsible for CTSS expression in proinflammatory macrophage. Western blot analysis (A) for CTSS expression in macrophage with increased concentrations of palmitic acid (PA, 0, 100, 200, 300, 400, 500 μM). Western blot (B) and qRT-PCR (C) analysis for the expression of IRF family gene expression under 500 μM PA. JASPAR (http://jaspar.genereg.net/) prediction of the IRF5 transcription binding site in the CTSS promoter (D). Dual-luciferase reporter system was performed with WT and MUT CTSS promoter with IRF5 overexpression (E). qRT-PCR and western blot analysis (F) for the efficiency of IRF5 gene knockdown in macrophage. An electrophoretic mobility shift assay (EMSA) was used to assess the IRF5 and CTSS interaction (G). qRT-PCR (H) and western blot analysis (I) for CTSS expression when transfected with scr or shIRF5-1. Data are shown as the mean ± SEM, n = 3, ANOVA. *P < 0.05, **P < 0.05

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