Fig. 2

Surgical method and fluorescence curve analysis. a Method of establishing an ischemia–reperfusion model in rat liver. The hepatic artery was ligated with 6–0 silk sutures, and the portal vein was clamped with microvessel clips; b Fluorescence intensity curve. Fluorescence intensity first grew fast, then slowed, and finally reached a plateau phase; c We chose the asymptotic regression model to fit the fluorescence intensity curve. Parameter b3, which represents the growth rate of the FI curve, decreased from groups I-VII; d Group A included groups I and II, group B included groups III-V, and group C included groups VI-VIII. Parameter b3 was significantly different among groups A, B and C (P < 0.01)