Fig. 4

Gene and protein expression of myosin (MHC) in injury site of external anal sphincter after laser, chondroitinase ABC (ChABC) and combination of laser and ChABC (Laser + ChABC) treatments. Myosin expression are observed in laser and ChABC groups (green). ***, represent significant level at p < 0.0001 with intact group. *, represent significant level at p < 0.05 with intact group. ###, represent significant level at p < 0.0001 with sphincterotomy group. ##, represent significant level at p < 0.01 with sphincterotomy group. $$, represent significant level at p < 0.01 with ChABC group. ††, represent significant level at p < 0.01 with laser group. Data are presented as means ± SEM (n = 3 animal per group). Real-time PCR and immunohistochemistry of external anal sphincter, rabbit (× 10)