Table 1 Primers used for TM6SF2, PCSK7, and PCSK9 polymorphisms typing
SNP | Primer (5′ → 3′) | Annealing temperature (°C) | Amplicon (bp) | Restriction enzyme | RFLP fragments (bp) |
|---|---|---|---|---|---|
TM6SF2-rs58542926 | F: CCAGGTGTTGTCTCAAGTGG R: CCAGGTGTTGTCTCAAGTGG | 60 | 247 | Hpy188I | T allele: 247 C allele: 175 + 71 |
PCSK7-rs2277287 | F: CATTGCCTAGGTATCCGGGT R: GGGCTTCTCATGTGGCAATC | 60 | 321 | PvuII | T allele: 321 C allele: 226 + 95 |
PCSK9-rs505151 | F: CACGGTTGTGTCCCAAATGG R: GAGAGGGACAAGTCGGAACC | 60 | 440 | Eam1104I (EarI) | G allele: 440 A allele: 290 + 150 |