Table 3 Estimation of NS5A and NS5B properties by “protparam”
PROTPARAM | NS5A 1a | NS5A 3a | NS5B 1a | NS5B 3a |
|---|---|---|---|---|
Number of amino acids | 229 | 233 | 591 | 591 |
Molecular weight | 25,637.51 | 25,565.16 | 65,311.27 | 66,134.92 |
Theoretical pI | 8.34 | 7.53 | 9.12 | 9.02 |
Estimated half-life | 1.9 h (mammalian reticulocytes, in vitro) | 1.9 h (mammalian reticulocytes, in vitro) | 1.9 h (mammalian reticulocytes, in vitro) | 1.9 h (mammalian reticulocytes, in vitro) |
> 20 h (yeast, in vivo) | > 20 h (yeast, in vivo) | > 20 h (yeast, in vivo) | > 20 h (yeast, in vivo) | |
> 10 h (Escherichia coli, in vivo) | > 10 h (Escherichia coli, in vivo) | > 10 h (Escherichia coli, in vivo) | > 10 h (Escherichia coli, in vivo) | |
Instability index | Unstable 45.71 | Unstable 44.48 | Unstable 41.41 | Unstable 45.66 |
Aliphatic index | 70.66 | 69.53 | 86.7 | 85.37 |
GRAVY | − 0.262 | − 0.226 | − 0.103 | − 0.219 |