Fig. 1
From: Sodium nitroprusside protects HFD induced gut dysfunction via activating AMPKα/SIRT1 signaling
SNP alleviated LPS-induced intestinal barrier dysfunction via AMPKα-SIRT1 signaling. Intestinal barrier dysfunction of Caco-2 cell monolayers was stimulated by 50 ng/mL LPS treatment for 46 h and LPS plus 10 µM/30 µM SNP treatment for 48 h. A–C Protein levels of intestinal barrier indicators ZO-1 and occludin. (D-F) Protein levels of phosphorylated AMPKα and SIRT1. G–I Protein levels of SIRT1 and phosphorylated AMPKα when AMPKα was knocked down. J–K Protein levels of occludin when AMPKα was knocked down; Data were presented as the mean ± S.E.M. (*p < 0.05, **p < 0.01, ***p < 0.001)