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Fig. 3 | BMC Gastroenterology

Fig. 3

From: SARS-CoV-2 identified by transmission electron microscopy in lymphoproliferative and ischaemic intestinal lesions of COVID-19 patients with acute abdominal pain: two case reports

Fig. 3

Complementary examinations (intestinal resection specimen, light microscopy and transmission electron microscopy) from case 1. A The ileocaecal resection specimen showed an ulcerated lesion with fibrosis of the intestinal wall, causing stenosis. B Haematoxylin and eosin staining of the surgical specimen showing diffuse large B-cell lymphoma germinal center subtype. Lymphocytes of medium and large size with irregular nuclei, vesicular chromatin, conspicuous nucleoli and mitotic figures were observed (×100). C- D Immunohistochemistry of the lesion was performed, showing expression for CD20, CD79a, bcl2, CD10, bcl6, LMO2, MUM1, and c-MYC. The tumour was classified as diffuse large B-cell lymphoma (DLBCL) germinal center subtype. In panel C, CD20 (clone L26, Ventana, Roche, Tucson, AZ, USA) immunohistochemistry is shown (×100). In panel D, a high proliferative index (Ki67) (clone 30-9, Ventana, Roche, Tucson, AZ, USA) was observed (90% in the hotspot areas) (100x). E- F Electron micrograph of the lymphoma tissue, in which viral particles are highlighted (red circles). E Viral particles (red circle) remained in occasional endothelial cells. F In contrast to coronavirus particles (red circle), pinocytotic vesicles (red arrow) have a smooth contour, their cell membrane lipid bilayer may be visualized, and they are often arranged in clusters

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