Fig. 2

XPro1595 treatment attenuates cerulein-induced pancreatic inflammatory infiltrates. Photomicrographs illustrate hematoxylin and eosin stained sections of mouse pancreas in non-cerulein (a, d) and cerulein (b, c, e, f) mice, 2 days after cerulein/vehicle administration. Vehicle-treated non-cerulein mice had an absence of inflammatory infiltrates (a) (n = 5), but cerulein treatment promoted an influx of inflammatory cells within 2 days (b) (n = 3), which had resolved by 7 days (c, d) (n = 4). XPro1595 treatment significantly reduced the immune cell infiltration at 2 days (e) (n = 3), that was even further reduced by 7 days (f) (n = 4). A week following pancreatitis induction the circulating macrophage population was also minimal in cerulein-treated mice, independent of vehicle or XPro1595 treatment (n = 4 each). C cerulein, V vehicle, XP XPro1595, **p < 0.01, scale bars = 100 µm