pH profile | Pepsin activity |
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pH measured every 15 s within the first 5 min; thereafter, every 5 min until the end of the 2-h incubation | 1) Cumulative activity: indirect measurement of digested amount of reference protein (BSA) after 0, 2.5, 5, 10, 15, 30, 45, 60, 90, 120 mina |
Time to reach pH 3.0, 3.5, 4.0, 4.5 Maximum pH and duration of highest pH value (-0.1 pH unit) | 2) Instantaneous activity: mathematical model, where pepsin activity was measured every 0.25 pH units from pH 1–7, using the same measurement of digested BSA as in 1)b |