Fig. 1
Effects of H2 pre-inhalation on pancreatic pathology (HE, × 400). a Representative pathological images of mouse pancreatic tissue. Acute pancreatitis (AP) model was induced by intraperitoneal injection of 50 μg/kg caerulein per hour for 6 injections in C57Bl/6 mice, and mice given normal saline (NS) injections at the same way as control. Some mice were treated with hydrogen-rich gases (42% H2 + 21% O2 + 37% N2) inhalation for 3 days before the first injection of caerulein or saline, while others still in normal air condition. NS: control mouse, HNS: control mouse given treatment of the hydrogen-rich gases pre-inhalation, AP: mouse with AP, HAP: mouse with AP and treated with the hydrogen-rich gases pre-inhalation; b, Pathological scores for edema, vacuolization, inflammation, and necrosis in each group. At least 15 randomly chosen microscopic fields from three slides of three mice in every group were examined and evaluated blindly. Results are presented as means ± SEM (n = 6). *p < 0.05 compared with the NS group at the same time point; #p < 0.05 compared with the AP group at the same time point