Fig. 2

Validation of the pre-pubertal chronic colitis model: histology. a Representative images of H&E from the Control group showing normal histology. b Representative images of H&E from the TNBS group showing severe transmural inflammation with glandular regeneration and severe fibrosis. c Representative images of H&E from the TNBS+MODULEN group showing severe inflammation and severe fibrosis. d Histological inflammation score by group. e Histological fibrosis score by group. f Colon weight/length ratio by group. *p < 0.05, **p < 0.01, significant comparisons are versus Control group unless otherwise specified. Panels d–f Control group: n = 8, TNBS group: n = 6, TNBS+Modulen group: n = 9