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Fig. 1 | BMC Gastroenterology

Fig. 1

From: Novel stromal biomarker screening in pancreatic cancer patients using the in vitro cancer-stromal interaction model

Fig. 1

Schematic explaining the study design strategy. Five primary cultivated fibroblasts from human pancreas were prepared and stimulated by two types of pancreatic CCCM (Capan-1 and MIA PaCa-2). Gene expression analysis of the fibroblasts was performed to elucidate up-regulated genes. Gene-expression data concomitant with overall survival in PDAC was collected from the TCGA and prognostic marker candidates were selected from these up-regulated genes. Prognostic marker candidates were confirmed to be expressed in 5 human fresh PDAC tissues compared to normal fresh tissues. Finally, survival analysis using immunohistochemistry on tissue microarrays consisting of 216 surgically resected PDAC tissues was performed. CCCM, cancer-cell-conditioned medium; PDAC, pancreatic ductal adenocarcinoma; TCGA, The Cancer Genome Atlas

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BMC Gastroenterology

ISSN: 1471-230X

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