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Fig. 6 | BMC Gastroenterology

Fig. 6

From: Carbon tetrachloride (CCl4) accelerated development of non-alcoholic fatty liver disease (NAFLD)/steatohepatitis (NASH) in MS-NASH mice fed western diet supplemented with fructose (WDF)

Fig. 6

Histopathology of Obeticholic acid (OCA, 30 mg/kg, QD) treatment on Western diet supplemented with fructose (WDF) fed MS-NASH or C57BI/6 mice under low dose CCl4 (0.08 mL/kg) twice weekly for 8 weeks. Left panel: Representative images of H&E and PSR staining in mice on WDF treated with CCl4. Yellow arrows indicate steatosis, blue arrows indicate typical microvesicular ballooning within the blue square, and red arrows indicate fibrosis. MS-NASH mice with (a and b) vehicle or (c and d) OCA. C57Bl/6 mice with (e and f) vehicle or (g and h) OCA. Bottom panel: Hepatosteatosis analysis from H&E images. (i) Semi quantification of Pathology scores (0–3), lobular inflammation (0–3), ballooning (0–2), and NAFLD activity (0–8). (j) Quantitative histology analyzed as percentage of steatosis area, and cell counts of inflammation and hepatic ballooning by Reveal ImageDx software. Right panel: Fibrosis analysis from PSR images and liver contents. (k) Fibrosis score by pathologist (0–4). (l) Fibrosis area by ImageDx. (m) Live hydroxyproline contents by biochemistry. Data represented as mean ± SEM. * p < 0.05, *** p < 0.005, Veh VS OCA group using Holm-Sidak t-test

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