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Fig. 4 | BMC Gastroenterology

Fig. 4

From: Carbon tetrachloride (CCl4) accelerated development of non-alcoholic fatty liver disease (NAFLD)/steatohepatitis (NASH) in MS-NASH mice fed western diet supplemented with fructose (WDF)

Fig. 4

Histopathology in Western diet supplemented with fructose (WDF) fed MS-NASH mice treated with low dose CCL4 (0.08 mL/kg) for 8 weeks. Left panel: Representative images of H&E and PSR staining from MS-NASH mice on WDF. Yellow arrows indicate macrovesicular vacuolation steatosis; blue arrows indicate typical microvesicular ballooning within the blue square; and red arrows indicate fibrosis. (a and b) Without CCl4. (c and d) With CCl4. Bottom panel: Hepatosteatosis analysis from H&E images. (e) Semi quantification of pathology scores (0–3), lobular inflammation (0–3), ballooning (0–2), and NAFLD activity (0–8). (f) Quantitative histology analyzed as percentage of steatosis area, and cell counts of inflammation and hepatic ballooning by Reveal ImageDx software. Right panel: Fibrosis analysis from PSR images and live contents. (g) Fibrosis score by pathologist (0–4). (h) Fibrosis area by ImageDx. (i) Live hydroxyproline contents by biochemistry. Data presented as mean ± SEM. *** p < 0.005, WDF vs. WDF + CCL4 group using Holm-Sidak t-test

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