Fig. 3

Effects of low dose CCL₄ (0.08 mL/kg) twice weekly for 8 weeks in MS-NASH mice fed Western diet supplemented with fructose (WDF). Top panel: (a) Body weights; serum (b) ALT and (c) AST levels before and after low dose CCL₄. In-life AST and AST levels at weeks 11, 12 and 14 were measured ~ 72 h; and the terminal one at week 16 measured ~ 24 h, after CCl₄ administration. Bottom Panel: Liver (d) weight; (e) cholesterol; and (f) triglycerides measured at the end of the study. Data presented as mean ± SEM. * p < 0.05, *** p < 0.005, WDF vs. WDF + CCl₄ group by Holm-Sidak t-test