Fig. 2
Histopathology in MS-NASH mice fed Control diet (CD) or Western diet supplemented with Fructose (WDF) treated high dose CCl4 (0.2 mL/kg) twice weekly for 3 weeks. Left panel: Representative images of H&E and PSR staining in animals fed (a and b) CD; and WDF treated (c, d) without or (e, f) with CCl4, respectively. Red arrows indicate fibrosis. Right panel: (g) Pathology scores of steatoses (0–3), lobular inflammation (0–3), ballooning (0–2), NAFLD Activity (0–8). (h) left: fibrosis score (0–4) and right: Percentage fibrosis area, quantitatively analyzed as total PSR positive staining area over total liver section area scanned and processed by HALO software. Data presented as mean ± SEM. # p < 0.05, ### p < 0.005 comparing with CD group; * p < 0.05, *** p < 0.005 comparing with WDF group by one-way ANOVA analysis