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Fig. 6 | BMC Gastroenterology

Fig. 6

From: Role of sex hormones and their receptors on gastric Nrf2 and neuronal nitric oxide synthase function in an experimental hyperglycemia model

Fig. 6

Effect of sex hormones and selective ER agonists (PPT, DPN) on Nrf2/nNOS, ERs, and HO-1 protein expression in 48 h in-vitro tissue culture. (a-c) The effect of 48 h in-vitro hyperglycemia was measured on nNOSα, Nrf2, ERα expression. In a separate set of experiments, the effect of PPT and DPN on (d-h) protein expression was assessed. Stripped blots were re-probed with β-actin. Data were normalized with housekeeping gene or protein (β-actin). Bar graphs showed a ratio of target gene or protein with β-actin. Data were analyzed using one-way ANOVA by using GraphPad prism software. Data are means ± SEM (n = 4). * p< 0.05 compared with NG groups. #  p< 0.05 compared with HG groups

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