Fig. 2

The positive criteria of FISH. Four or more cells with DNA gains at chromosomes 3, 7, and 17 (a) with or without loss of locus 9p21(b); more than four cells with polyploidy of only one chromosome (c) accompanied by loss of 9p21(d); and more than 12 cells with loss of 9p21(f) without gain of chromosomes 3, 7, and 17(e). Normal diploid cells were considered negative (e and b). Green signals for chromosome 3, aqua signals for 7, red signals for chromosome 17 in trichromic set and green signals for chromosome 3, red signals for 9p21 in double chromic set