Fig. 4

GAN DIO-NASH mice develop characteristics of the metabolic syndrome. Terminal body weight (a), whole-body fat mass (b), whole-body lean mass (c), liver weight (d). **p < 0.01, ***p < 0.01 (unpaired t-test; chow-fed mice, n = 6–10; GAN DIO-NASH mice, n = 14–16). An intraperitoneal glucose tolerance test (ipGTT) was performed one week before termination. (e) Glucose excursion curves (− 60 to 180 min). ***p < 0.001 (repeated-measure two-way ANOVA; chow-fed mice, n = 10; GAN DIO-NASH mice, n = 15). Insert, AUC-glucose levels (0–180 min). **p < 0.01 (unpaired t-test). Terminal plasma insulin (f) and HOMA-IR (g). ***p < 0.01 (unpaired t-test; chow-fed mice, n = 10; GAN DIO-NASH mice, n = 15). Terminal plasma leptin (h), total cholesterol (i), triglycerides (j) and free fatty acids (k). **p < 0.01, ***p < 0.01 (unpaired t-test; chow-fed mice, n = 5–10; GAN DIO-NASH mice, n = 8–15)