Fig. 2

CD44v8–10 showed up-regulation in CRC at the expense of other CD44 splicing variants. a Diagram of the method to calculate PSI_junc5’, which represents the usage of a junction among all junctions sharing the same 5’ splice site. The boxes are exons. Thick bars connected by a dotted line represent a read cover two exons (junction read). a, b and c are read counts for the three junctions. b Similar to (a) except that diagram of PSI_junc3’ was shown, which represents the usage of a junction among all junctions sharing the same 3’ splice site. c CD44 gene structure (bottom) and read coverage (Sashimi Plot) for exon 5 to exon 16 regions. d Boxplot of PSI of CD44 junc_5’ E5-v8 (top row), junc_5’ E5-E16 (middle row) and junc_3’ v10-E16 (bottom row) in CRC18P (left column) and CRC10P (right column) datasets. Dots in the boxplot represent individual patient data. P-value is based on Wilcoxon rank-sum test