Fig. 1

Cassette exon regulation in CRC. a Diagram of the method to calculate the PSI of an exon. The orange boxes and gray boxes are the alternative exons and neighboring exons. Thick bars connected by a dotted line represent a read cover two exons (junction read). a, b and c are read counts for the three junctions. b Venn diagram showing the overlap of exon splicing events between CRC and NC in CRC18P and CRC10P datasets. P-value of Wilcoxon rank-sum test< 0.05 and |ΔPSI| > 20% were used as cutoffs to select significant events. Inc., exon inclusion in CRC; Exc, exon exclusion in CRC. c CLK1 gene structure (top) and read coverage (Sashimi Plot) for exon 3 to exon 5 region. d Boxplot of PSI of CLK1 exon4 in the CRC18P dataset. Dots in the boxplot represent individual patient data. P-value is based on Wilcoxon rank-sum test. e Similar to (d) except that the CRC10P dataset was shown. f COL6A3 gene structure (top) and read coverage (Sashimi Plot) for exon 5 to exon 7 regions. g Boxplot of PSI of COL6A3 exon6 in the CRC18P dataset. Dots in the boxplot represent individual patient data. P-value is based on Wilcoxon rank-sum test. h Similar to (g) except that the CRC10P dataset was shown