Fig. 4

MiR-15b regulates EMT-related proteins via targeting DCLK1. a The binding site of miR-15b to DCLK1 was analysed using bioinformatics prediction software. b GC cells were co-transfected with either miR-15b mimic or NC mimic and pmirGLO Dual-Luciferase miRNA Target Expression Vector with WT or MUT 3′-UTR of DCLK1. Relative luciferase activity was determined by a dual-luciferase assay system. c The relative expressions of DCLK1 in NC, miR-15b mimics and miR-15b inhibitor were detected by qRT-PCR. d The expressions of DCLK1, Notch1 and EMT-related proteins in NC, miR-15b mimic and miR-15b inhibitor were detected by Western blotting. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001 compared with the NC group