A case report of a novel 22 bp duplication within exon 1 of the UGT1A1 in a Sudanese infant with Crigler-Najjar syndrome type I

Table 1 UGT1A1 gene primers used in PCR and Sequencing

Region	Primer Name	Primer sequence	Annealing Temp.	Amplicon size bp
Promoter	UGT1A1- P-F	AACTCCCTGCTACCTTTGTGGA	60	473
	UGT1A1- P-R	TGATCACACGCTGCAGGAAAGA
Exon1	UGT1A1-E1A-F	AGGAGCAAAGGCGCCATGGCT	60	528
	UGT1A1-E1A-R	GAAGAATACAGTGGGCAGAGAC
	UGT1A1-E1B-F	CATGCTGACGGACCCTTT	54	588
	UGT1A1-E1B-R	GATGCCAAAGACAGACTCAAAC
Exon 2	UGT1A1-E2-F	CTCAAACACGCATGCCTTTAAT	54	322
	UGT1A1-E2-R	GAAGCTGGAAGTCTGGGATTAG
Exon 3	UGT1A1-E3-F	AAGACTGTTCCTTCAGAGGAC	58	283
	UGT1A1-E3-R	AGCTCAACAATCCTTTAGAATAGC
Exon 4	UGT1A1-E4-F	TGCAAGGGCATGTGAGTAA	56	432
	UGT1A1-E4-R	GAAACAACGCTATTAAATGCTAG
Exon 5	UGT1A1-E5-F	GCCATGAGCATAAAGAGAGGAT	60	597
	UGT1A1-E5-R	CCTGATCAAAGACACCAGAGG

PCR conditions: PCR reaction mixture (10 μL) contained 1 µL of genomic DNA, 0.25 μL of each forward and reverse primer (diluted to 10 μmol/L), 3.5 μL of ddH2O, 5 μL of 2 × Taq PCR Master Mix (Emerald GT mix, Clontech). PCR was implemented by denaturation at 94 °C for 10 s, followed by 40 thermal cycles composed of 98 °C for 30 s, 60 °C(promoter) for 30 s, and 72 °C for 1 min, with a final extension at 72 °C for 10 min

ISSN: 1471-230X