Fig. 3

MiR-149-5p mediated the regulatory effects of uric acid on lipid accumulation in hepatocytes. a Real-time PCR confirmed miR-149-5p mimic increased miR-149-5p expression in AML-12 cells. b Overexpression of miR-149-5p aggregated uric acid-induced intracellular triglyceride accumulation in AML-12 cells. c Real-time PCR confirmed that miR-149-5p inhibitor decreased miR-149-5p expression in AML-12 cells. d Inhibition of miR-149-5p alleviated uric acid-induced intracellular triglyceride accumulation in AML-12 cells. e Oil Red O staining conformed the regulatory roles of miR-149-5p on uric acid-induced hepatocytes lipid accumulation (× 200). Data are presented as the mean ± SD of at least three independent replicates. * P < 0.05, ** P < 0.01, *** P < 0.001 of two-tailed student’s t-test