Fig. 1
Mice that lack Gankyrin (GK) levels in epithelial cells in the upper small intestine are hypersusceptible to dextran sodium sulfate (DSS)-induced colitis. (A) Representative images of hematoxylin and eosin stained colonic tissues in Gankyrinf/f (GKf/f), Cdx2-Cre;Gankyrinf/f (Cdx2-Cre;GKf/f) and Villin-Cre;Gankyrinf/f (Villin-Cre;GKf/f) mice. Cdx2-Cre;GKf/f, Villin-Cre;GKf/f and GKf/f mice were administered with 2.5% DSS in drinking water for 7 days. Scale bar, 100 μm. (B) Epithelial injury and inflammatory cell infiltration into colonic tissues of GKf/f, Cdx2-Cre;GKf/f, and Villin-Cre;GKf/f mice were observed after 7 days of the initiation of DSS administration. n = 6 per group. (C) RNA was extracted from colonic tissues of DSS-treated Villin-Cre;GKf/f (n = 6), Cdx2-Cre;GKf/f (n = 4), and GKf/f (n = 5) mice. Relative quantities of mRNA were determined by real-time qPCR and were normalized to the quantity of β-actin mRNA. The relative quantity of each mRNA in the untreated colon was assumed to be an arbitrary value of 1.0. Data are represented as mean ± SEM. (D) Body weight of DSS-treated Villin-Cre;GKf/f (n = 6), Cdx2-Cre;GKf/f (n = 4), and GKf/f (n = 5) mice were shown at the time point of day 0 and day 7