Fig. 1

Visualization of stereological sections and morphometric analysis. Schematic drawing of liver lobes used for stereology (a); left lateral lobe (1), including site of pre-biopsy (red), post biopsy (black square), and stereology section (half the lobe marked by the dashed line), medial lobe (2), and right lateral lobe (3), as well as the caudate lobe (4) and papillary process (5). Morphometric analysis of; steatosis (b) on hematoxylin and Eosin Y stained sections, as well as immunohistochemistry (IHC) of galectin-3 (gal-3) (c), and of type I collage α1 chain (col1a1) (d). Pink is assigned to steatosis, dark grey assigned to liver tissue, yellow assigned to gal-3 positive stain, and green assigned to col1a1 positive stain. The morphometrical assessments are performed using Visiomorph Software (Visiopharm, Hoersholm, Denmark), by pixel annotation, excluding vessels based on size (white is assigned to vessels), as well as excluding gal-3 and col1a1 positive stain surrounding vessels. Scale bar = 150 μm