Fig. 1

The comparative effects of different diets on liver histology to evaluate fatty change (a), inflammation (b), ballooning degeneration (c) (kruskal-wallis followed by mann-whitney’s test), and NAS score (d) (one-way ANOVA followed by tukey’s test) in hepatic tissues of mice fed with SD, Fru, HFD, and WD with or without cigarette exposure after 8 weeks with hematoxylin and eosin staining using the semiquantitative NAS System. Data are depicted using box and whisker plots showing median, minimum and maximum values, n = 8 in each group. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001 significant difference compared to mice fed standard diet as control. ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001significant difference compared to smoker mice fed WD. c: control; Cs: Cigarette smoke; Fru: fructose; HFD: high fat diet; WD: western diet; NAS: NAFLD Activity Scoring