| Denaturation | Extension | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Temperature | Time | Temperature | Time | ||||||||
 | Denaturation | Annealing | Extension |  | |||||||
Temp | Time | Temp | Time | Temp | Time | No. cycles | |||||
G typing 1st round | 94 | 5 | 94 | 1 | 42 | 2 | 72 | 1 | 35 | 72 | 7 |
2nd round | 94 | 5 | 94 | 1 | 45 | 2 | 72 | 1 | 15 | 72 | 7 |
P typing * 1st round | 94 | 5 | 94 | 1 | 52 | 1 | 72 | 1 | 35 | 72 | 7 |
2nd round | 94 | 5 | 94 | 1 | 52 | 1 | 72 | 1 | 20 | 72 | 7 |