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Table 2 G and P typing PCR programs

From: Molecular and serologic characterization of rotavirus from children with acute gastroenteritis in northern Iran, Gorgan

 

Denaturation

Extension

Temperature

Time

Temperature

Time

 

Denaturation

Annealing

Extension

 

Temp

Time

Temp

Time

Temp

Time

No. cycles

G typing 1st round

94

5

94

1

42

2

72

1

35

72

7

2nd round

94

5

94

1

45

2

72

1

15

72

7

P typing * 1st round

94

5

94

1

52

1

72

1

35

72

7

2nd round

94

5

94

1

52

1

72

1

20

72

7