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Table 2 G and P typing PCR programs

From: Molecular and serologic characterization of rotavirus from children with acute gastroenteritis in northern Iran, Gorgan

  Denaturation Extension
Temperature Time Temperature Time
  Denaturation Annealing Extension  
Temp Time Temp Time Temp Time No. cycles
G typing 1st round 94 5 94 1 42 2 72 1 35 72 7
2nd round 94 5 94 1 45 2 72 1 15 72 7
P typing * 1st round 94 5 94 1 52 1 72 1 35 72 7
2nd round 94 5 94 1 52 1 72 1 20 72 7