Table 1 VP7 and VP4 consensus and type-specific primers
Primer | Type | Sequence (5′→3′) | Position | PCR product (bp |
|---|---|---|---|---|
Universal primer | ||||
 VP7 con1 Forward |  | ATGTATGGTATTGAATATACCAC | 51–71 | 1014 |
 VP7 con2 Reverse |  | GGTCACATCATACAATTCTAATC | 1062–1040 |  |
 VP4 con1 Forward |  | TGGCTTCGCTCATTTATAGACA | 2–23 | 877 |
 VP4con1 Reverse |  | ATYTCHGACCAYTTATAHCC | 878–859 |  |
G-typing (VP7) | ||||
 Gcon Reverse |  | GGTCACATCATACAATTCT | 1062–1044 |  |
 aBT1 | G1 | CAAGTACTCAAATCAATGATGG | 314–335 | 749 |
 aCT2 | G2 | CAATGATATTAACACATTTTCTGTG | 411–435 | 652 |
 Aust | G3 | ACGAACTCAACACGAGARG | 250–269 | 813 |
 aDT4 | G4 | CGTTTCTGGTGAGGAGTTG | 480–498 | 58 |
 aAT8 | G8 | GTCACACCATTTGTAAATTCG | 178–198 | 88 |
 mG9 | G9 | CTTGATGTGACTAYAAATAC | 757–776 | 305 |
P-typing (VP4) | ||||
 con3 Forward |  | TGGCTTCGCTCATTTATAGACA | 11–32 |  |
 2 T-1 | P [4] | CTATTGTTAGAGGTTAGAGTC | 474–494 | 484 |
 3 T-1 | P [6] | TGTTGATTAGTTGGATTCAA | 259–278 | 260 |
 1 T-1 | P [8] | TCTACTTGGATAACGTGC | 339–356 | 346 |
 4 T-1 | P [9] | TGAGACATGCAATTGGAC | 385–402 | 392 |