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Table 5 The quality of 16S rRNA sequencing of fecal bacteria in patients with ulcerative colitis

From: Fecal microbiota transplantation for ulcerative colitis: a prospective clinical study

Sample ID Raw Reads Combined Raw Tags Qualified Raw Tags Effective Bases (nt) Q30 (%) GC (%) Effective(%)
d0-A3 93,040 88,487 79,811 30,944,428 97.16 52.49 80.8
d0-A5 83,603 79,229 70,709 27,506,095 97.19 51.77 78.56
d0-A6 97,570 91,686 82,546 31,275,447 97.18 50.58 76.69
d0-A8 86,502 81,642 73,450 27,695,120 97.17 51.43 76.82
d0-A10 81,604 77,275 70,049 26,399,958 97.15 51.3 78.02
d0-A11 98,275 92,745 83,720 32,698,787 97.13 51.45 79.48
d0-A12 85,580 77,340 60,971 22,690,822 94.2 51.9 63.55
d0-A14 89,314 84,745 76,866 28,211,684 97.22 52 76.51
d0-B1 91,830 87,008 76,747 30,617,125 96.95 53.2 79.9
d0-B2 82,081 77,921 67,290 27,251,920 96.81 54.73 77.99
d0-B4 91,046 86,561 77,753 30,224,143 97.1 53.54 80.6
d0-B7 88,594 83,843 72,949 28,241,953 96.94 52.89 75.45
d0-B9 89,766 85,407 77,936 28,937,019 97.21 51.59 78.3
d1-A3 94,602 89,947 80,540 31,277,847 97.13 52.55 80.08
d1-A5 88,810 83,587 75,024 29,506,841 97.12 51.8 79.36
d1-A6 92,458 87,430 78,588 30,928,414 97.13 51.74 80.44
d1-A8 88,997 83,816 74,889 28,211,660 97.14 51.58 75.6
d1-A10 95,021 89,688 80,983 30,012,662 97.09 51.25 76.24
d1-A11 88,286 83,310 75,278 28,145,749 97.16 51.5 76.35
d1-A12 97,742 88,876 68,748 26,725,630 94.22 53.22 65.16
d1-A13 86,350 81,399 72,066 27,885,218 97.04 52.02 76.87
d1-B1 84,009 79,994 72,638 27,442,698 97.22 52.26 79.48
d1-B2 92,523 88,004 76,896 31,318,550 96.88 54.09 79.79
d1-B4 99,557 94,523 83,760 33,197,434 97.05 52.82 79.67
d1-B7 81,447 76,527 68,433 25,102,970 97.1 51.84 73.74
d1-B9 85,819 81,046 72,942 28,034,477 97.07 51.26 78.23
d2-A8 86,695 82,017 73,730 26,868,038 97.15 52.25 74.5
d2-A12 82,621 78,323 69,553 26,127,370 97.04 51.21 75.78
d2-B2 85,964 81,497 71,186 28,547,029 96.92 53.42 78.78
d2-B7 81,000 76,000 67,820 24,654,602 97.05 51.4 72.8
  1. Summary of columns:
  2. (1) Sample ID: sample name
  3. (2) Raw Reads: the number of PE-Reads in the original machine
  4. (3) Combined Raw Tags: the spliced tags sequence
  5. (4) Qualified Raw Tags: tags sequence for subsequent analysis after quality control filtering
  6. (5) Effective Bases (nt): the number of bases of the effective tags
  7. (6) Q30 (%): the percentage of bases with a base quality value greater than 30 (sequencing error rate < 0.1%) in effective tags
  8. (7) GC (%): the content of GC base in the effective tags
  9. (8) Effective Rate (%): The ratio of effective tags to raw PE-reads