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Fig. 6 | BMC Gastroenterology

Fig. 6

From: The Ussing chamber system for measuring intestinal permeability in health and disease

Fig. 6

T-regulatory cell depleted mice (DTx) show increased intestinal permeability. a Representative FACS plots showing depletion of FoxP3+ cells. b Percentage body weight and (c) colon length of untreated and treated mice. d Haematoxylin and eosin staining of distal colonic sections from untreated and treated animals. Asterisks and arrows indicate increased immune cell infiltration and disrupted crypt structure respectively. e TER and (f) 4 kDa FITC-dextran passage of proximal and mid colonic sections. Mucosal starting concentration was 1 mg/ml. n = 3 treated vs 3 untreated 16-week-old female mice. Unpaired students T tests and a Mann Whitney test were used for comparisons in (e) and (f) respectively. Significant differences indicate: *P < 0.05, **P < 0.001, ***P < 0.001. Error bars show +/− SEM

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