Fig. 2

Mouse dissection and mounting technique. a Midline incision showing exposed abdominal organs. Black dotted lines indicate dissection points to obtain the intestine during the procedure. b Colon is flushed with Krebs buffer and opened longitudinally into a flat sheet using micro-dissecting scissors. X-ray film squares with the desired aperture are applied to the proximal, mid or distal regions. A second x-ray film with corresponding aperture size is placed on top, sandwiching tissue in-between. c Human biopsy sample showing contraction of serosal tissue leads to formation of a crescent morphology with the mucosal surface facing upwards. Figures are original, created using Microsoft PowerPoint for Mac version 16.20