Table 1 Primer sequences for PCR detection of virulence genes
Target gene | Primer pair (5′-3′) | Amplicon length (bp) | Control strains | References |
|---|---|---|---|---|
cagA | F: ACCGCTCGAGAACCCTAGTCGGTAATGGG R: CAGGTACCGCGGCCGCTTAAGATTTTTGGAAACCAC | 981 | J99 | [25] |
vacAs1/S2 | F: ATGGAAATACAACAAACACAC R: CTGCTTGAATGCGCCAAAC | 259/286 | J99/Tx30a | |
vacAm1 | F: GGTCAAAATGCGGTCATGG R: CCATTGGTACCTGTAGAAAC | 290 | J99 | |
vacAm2 | F: CATAACTAGCGCCTTGCAC R: GGAGCCCCAGGAAACATTG | 352 | B8 | |
dupA | F: GACGATTGAGCGATGGGAATAT R: CTGAGAAGCCTTATTATCTTGTTGG | 971 | P12/J99 | [26] |