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Table 2 Antibodies

From: In-depth characterization of the Wnt-signaling/β-catenin pathway in an in vitro model of Barrett’s sequence

  Dilution Company Catalogue number
Primary antibodies
 pGSK3β-Ser9 1:1000 (1% BSA/PBS) Cell Signalling Technology, Danvers, USA 5558
 pβ-catenin-Ser552 1:1000 (1% BSA/PBS) Cell Signalling Technology, Danvers, USA 5651
 pAkt-Ser473 1:1000 (1% BSA/PBS) Cell Signalling Technology, Danvers, USA 4060
 GSK3β 1:1000 (1% BSA/PBS) Cell Signalling Technology, Danvers, USA 12456
 β-catenin 1:1000 (1% BSA/PBS) Cell Signalling Technology, Danvers, USA 8480
 Akt 1:1000 (1% BSA/PBS) Cell Signalling Technology, Danvers, USA 4691
 β-Actin 1:2500 (0.5% LFM/PBS) Sigma-Aldrich, Taufkirchen, Germany A1978
Secondary antibodies
 Goat anti-rabbit 1:7500 (0.5% LFM) Jackson Immuno Research, Suffolk, UK 111–035-045
 Goat anti-mouse 1:7500 (0.5% LFM). Jackson Immuno Research, Suffolk, UK 115–035-068
  1. BSA: bovine serum albumin; LFM: low fat milk; PBS: phosphate buffered saline