Fig. 1From: In-depth characterization of the Wnt-signaling/β-catenin pathway in an in vitro model of Barrett’s sequenceExpression of the extracellular ligands Wnt3a and Wnt5a along Barrett’s sequence. Expression of Wnt3a (a) and Wnt5a (b) was analyzed in EPC-1 and EPC-2 (squamous esophageal epithelium), CP-A (metaplastic epithelium), CP-B (epithelium with high-grade dysplasia), OE33 and OE19 (esophageal adenocarcinoma) cells by quantitative Real time RT-PCR. Highest Wnt3a expression was detected in EPC-1 and EPC-2 (a). Wnt5a was also expressed in EPC-1 and EPC-2, but highest levels of Wnt5a were found in CP-B (b). OE33 and OE19 expressed only marginal levels of Wnt3a and Wnt5a (a, b). Normalization was done with β-Actin. Values are shown as mean ± S.E.M. (One-way-ANOVA with Bonferroni correction, * - p < 0.05, ** - p < 0.01, *** - p < 0.001 compared to EPC-1)Back to article page