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Table 2 Overview of primary and secondary antibodies used in immunocytochemistry

From: Neuroprotective effects of vitamin D on high fat diet- and palmitic acid-induced enteric neuronal loss in mice

Raised against

Dilution

Code

Source

Host

References

Human neuronal protein, (HuC/HuD)

1:600

A21272

ThermoFisher Scientific, SE

Mouse

[37, 73]

Human gene product 9.5, (PGP 9.5), purified human brain

1:1.200

RA95101

Ultraclone, UK

Rabbit

[73]

PDIA3

1:500

AF8219

R&D systems, USA

sheep

 

VDR

1:400

Sc-13133

Kind gift professor BO Nilsson, Lund

Mouse

[74]

S100B (S100), purified bovine brain

1:12.000

Z0311

DAKO, DK

Rabbit

[75]

Alexa Fluor 488 anti-mouse IgG

1:1.000

115-485-166

Jackson Lab Inc, USA

Goat

 

Dy-light 594 anti-mouse IgG

1:1.000

715-515-151

Jackson Lab Inc, USA

Donkey

 

Alexa Fluor 594 anti- rabbit IgG

1:1.000

711-515-152

Jackson Lab Inc, USA

Donkey

 

Alexa Fluor 488 anti goat IgG

1:1000

705-545-147

Jackson Lab Inc, USA

donkey

 

Texas Red anti goat IgG

1:400

705-075-147

Jackson Lab Inc, USA

donkey

Â