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Fig. 4 | BMC Gastroenterology

Fig. 4

From: Neuroprotective effects of vitamin D on high fat diet- and palmitic acid-induced enteric neuronal loss in mice

Fig. 4

Effects of experimental treatment agent per se and on VD and VD+PA neuronal survival and PA-induced neuronal loss in primary cultures of myenteric neurons. a Supplementation with the protein disulphide isomerase family A member 3 (PDIA3) inhibitor 16F16 in the range 7x10-8M - 7x10-7M did not affect neuronal survival, but induced loss of all cells at 2x10-5 M. b Supplementation with the PDIA3 inhibitor (10-7M) had no effect on the palmitic acid (PA, 4x10-4M), the 1α,25-hydroxy-vitamin D3 (VD, 10-7M) or the PA+VD effects on neuronal survival. c Peroxisome proliferator-activated receptor gamma (PPARγ) inhibitor, GW69662 (10-6M) did not prevent the PA-induced neuronal loss but prevented the VD-induced prevention of the PA-induced loss. d Supplementation with pre-immune sera (1:250) had no effects on PA-, VD- or PA+VD-induced effects on neuronal survival. e Supplementation of isocitrate lyase (ICL) immune sera (1:250) did not prevent the PA-induced loss but did prevent the protective effects of VD on PA-induced loss. Untreated controls were run in parallel. Data presented as mean ± SEM, control n=6-18 per treatment agent, PDIA3 inhibitor 16F16 n=6-12, PPARγ inhibitor, GW69662 n=6, pre-immune sera n=6, ICL immune sera n=6, PDIA3 inhibitor (10-7M) + PA/VD/VD+PA n=6, PPARγ inhibitor (10-6M) + PA/VD/VD+PA n=6-12, pre immune sera (1:250) n=12, ICL immune sera (1:250) n=12-18, ** p< 0.01, *** p<0.001

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