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Fig. 3 | BMC Gastroenterology

Fig. 3

From: Neuroprotective effects of vitamin D on high fat diet- and palmitic acid-induced enteric neuronal loss in mice

Fig. 3

Localisation of PDIA3 in mouse and primary cultures of myenteric neurons. Representative micrographs of ileum of normal diet 1x vitamin D animals (a-c, g-i) and of primary cultures myenteric neurons (d-f, j-l). a-f Immunocytochemical staining of neuronal cell bodies and fibres using PGP9.5 in ileum (a) and culture (d) and PDIA3 positive fibres and cell bodies in ileum (b) and culture (e), merged images showing PDIA3 is not co-located with enteric neurons in ileum (c) and culture (f). g-i Immunocytochemical staining to enteric glia using S100β in ileum (g) and culture (j) and PDIA3 positive fibres and cell bodies in ileum (h) and culture (k), merged images show co-localisation of S100β and PDIA3, suggesting PDIA3 is expressed in enteric glia. Bars represent 20μm

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