Fig. 5

Apatinib inhibits VEGF- induced cell invasion. a Representative images of transwell (up) and quantification of invasion cell number (bottom). si-Control and and si-KDR cells grown in six-well plates were scratched and treated with PBS, VEGF (100 ng/ml), or VEGF (100 ng/ml) combined with apatinib (100 nM) for 24 h. Data are representative of three independent experiments. **P < 0.01. b Protein expression of Slug, snail and MMP9 in si-Control and si-KDR cells when treated with 100 ng/ml rhVEGF or 100 ng/ml rhVEGF + 100 nM apatinib for 24 h. GAPDH was detected as reference. **P < 0.01