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Fig. 2 | BMC Gastroenterology

Fig. 2

From: Apatinib affect VEGF-mediated cell proliferation, migration, invasion via blocking VEGFR2/RAF/MEK/ERK and PI3K/AKT pathways in cholangiocarcinoma cell

Fig. 2

Effects of si-KDR or si-Control on rhVEGF-induced VEGFR2 expression in QBC939 and TFK-1 cells.QBC939 and TFK-1 cells were treated with 0, 20, 50, 100 and 200 ng/ml of rhVEGF for 2 h and obtained from another 24-h incubation in medium, the mRNA level (a) and protein expression of VEGFR2 (b) was detected. GAPDH was detected as reference. c Relative cell viability of QBC939 and TFK-1 cells post 100 ng/ml rhVEGF treatment compared to control group. Data shown are means ± SD (n = 3). *P < 0.05 in QBC939 and TFK-1 cells versus control group (not treatment with 100 ng/ml rhVEGF). d Protein expression of VEGFR2 in siKDR group with or without 100 ng/ml rhVEGF treatment. GAPDH was detected as reference. Data are representative of three independent experiments. **P < 0.01, ***P < 0.001

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