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Fig. 1 | BMC Gastroenterology

Fig. 1

From: Apatinib affect VEGF-mediated cell proliferation, migration, invasion via blocking VEGFR2/RAF/MEK/ERK and PI3K/AKT pathways in cholangiocarcinoma cell

Fig. 1

VEGFR2 expression in QBC939 and TFK-1 cells transfected with siKDR or siControl. a Cells were transfected with 50 nM siKDR or siControl for 48 h. qRT-PCR was performed to evaluate the mRNA level of VEGFR2 Data shown are means ± SD (n = 3). **P < 0.01 and * P < 0.05 in QBC939 and TFK-1 cells versus si-Control group, respectively. b Protein expression of VEGFR2 in transfected QBC939 and TFK-1 cells were also detected by western-blotting. GAPDH was detected as reference. c Densitometric analysis of the autoradiographic plaques of these proteins is shown on the Fig. 1b. Data shown are means ± SD (n = 3). **P < 0.01 in QBC939 and TFK-1 cells versus si-Control group

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