Fig. 5

The roles of SPARCL1 knockdown in GIST 882 cell proliferation, cell cycle, apoptosis, migration and invasion. a, b Downregulation of SPARCL1 in GIST cells was confirmed by RT-qPCR and western blot. ▲compared with RNAi-3, P < 0.05; * compared with RNAi-2, P < 0.05. c: A CCK-8 assay was performed to evaluate the influence of SPARCL1 knockdown on GIST 882 cell proliferation. d: SPARCL1 downregulation could induce the arrest of cell cycles in G0/G1 phase. ▲ * compared with the other two groups, both P < 0.05. e SPARCL1 downregulation did not affect apoptosis of GIST 882 cell. f Wound-healing assay showed that SPARCL1 knockdown promoted migration of GIST 882 cells after cultured for 24 h. * compared with the other two groups, both P < 0.05. g The invasive GIST cells and quantitation of invasive GIST cell counts. * compared with the other two groups, both P < 0.05. Data represent the mean of three independent experiments, and the error bars refer to SD from the mean